Abstract—The aim of this study was to screen different carbon sources for Staphylococcus aureus to identify potential replacements for glucose. Glucose is a commonly used carbon source in microbial culture media, however, it can be expensive and may not be readily available in certain regions. In this study, a total of 6 different carbon sources were tested, including lactose, maltose, sucrose, fructose, glucose, n-hexane, toluene. The growth rate and biomass production of S. aureus were compared between each carbon source and glucose, which served as the control. The results showed that S. aureus was able to grow on all of the tested carbon sources, however, there were significant differences in the growth rates and biomass production between each carbon source. Specifically, fructose were found to be the most promising replacements for glucose, as they were able to support similar growth rates and biomass production as glucose.