Abstract—Milk is a highly nutritious commodity. The presence of all types of nutrients at a neutral pH brings about a favourable environment for the growth of various microorganisms, including bacteria, fungi, and molds. In this study, raw milk from 10 buffalos and 10 cows was collected from a dairy station in Limda, Gujarat, India. The milk was obtained from containers. These samples were then used to isolate various bacteria present. Using culture-dependent techniques, bacteria were isolated and were checked for their resistance to common antibiotics in the market. Amoxycillin-clavulanic acid, azithromycin and clindamycin were selected to verify the antibiotic resistance of these bacteria. Amoxycillin-clavulanic acid and azithromycin were in the form of tablets. Clindamycin was in the form of 1% gel. Antibiotic solutions were prepared and susceptibility testing was done using disk diffusion method. The results showed that over 85% of the 20 samples were resistant to clindamycin, 10% were resistant to azithromycin, and all samples were sensitive to amoxycillin-clavulanic acid. Further identification of these bacterial species is required to assess the major bacterial species in each sample.