Abstract—This study investigates the physical fitness levels of sportsmen and non-sportsmen through the administration of four standardized fitness tests: the 600-yard Walk-Run Test (Endurance), Standing Broad Jump (Leg Strength), 50-meter Dash (Speed), and 4x10 Meter Shuttle Run (Agility). These tests were carefully designed to measure key fitness parameters and identify variations between the two groups. The study outlines detailed procedures for each test, ensuring consistency and safety during administration. Results were systematically recorded and compared to highlight the differences in cardiovascular endurance, muscular strength, sprinting speed, and agility. The findings underscore significant variations in physical fitness metrics, offering valuable insights into the impact of athletic training on overall fitness levels. This paper also provides practical guidelines for administering physical fitness tests, ensuring reliability and reproducibility across diverse participant groups.